培養(yǎng)基配方

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Tomato Dextrin Yeast Medium

[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-9-17] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]

山東拓普生物工程有限公司培養(yǎng)基配方 http://521google.com.cn

Composition per liter:

Tomato paste............................................................................... 20.0g

Dextrin ........................................................................................ 20.0g

Agar ............................................................................................ 20.0g

Baker’s yeast............................................................................... 10.0g

CoCl2·6H2O ............................................................................... 5.0mg

pH 7.2–7.4 at 25°C

Preparation of Medium: Add components, except agar, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 7.2–7.4. Add agar. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Streptomyces avermitilis.

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