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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acid Egg Medium
Composition per 1640.0mL:
Potato starch................................................................................30.0g
KH 2 PO 4 .......................................................................................12.3g
Malachite Green............................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.3g
Penicillin G.........................................................................100,000IU
Fresh egg mixture.........................................................................1.0L
Glycerol ...................................................................................12.0mL
Source:  This medium is available as a prepared medium from Oxoid
Unipath.
Preparation of Medium: Add components to 1.0L of fresh egg
mixture. Mix thoroughly. Gently heat and bring to boiling. Bring vol-
ume to 1640.0mL with distilled/deionized water. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C with tubes in
an upright position.
Use:  For the cultivation and maintenance of Mycobacterium tubercu-
losis.