[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-4-16] [發(fā)布人:李佳頤] [閱讀次數(shù):] [返回]

Horse Serum Broth

山東拓普生物工程有限公司 培養(yǎng)基配方 http://521google.com.cn

Composition per liter:

Pancreatic digest of gelatin...........................................................5.0g
Beef extract...................................................................................3.0g
Horse serum...........................................................................200.0mL
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 800.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse se-
rum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Pseudomonas aeruginosa
and Streptobacillus moniliformis.