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培養(yǎng)基配方
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Yersinia Selective Agar Base
[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-12-29] [發(fā)布人:網(wǎng)站管理員2] [閱讀次數(shù):] [返回]
Yersinia Selective Agar Base
山東拓普生物工程有限公司 培養(yǎng)基配方 http://521google.com.cn
Composition per liter:
Mannitol............................................................20.0g
Peptone..............................................................17.0g
Agar ..................................................................12.5g
Proteose peptone.................................................3.0g
Yeast extract........................................................2.0g
Sodium pyruvate.................................................2.0g
NaCl....................................................................1.0g
Sodium deoxycholate.........................................0.5g
MgSO4·7H2O ....................................................0.01g
Neutral Red.......................................................0.03g
Crystal Violet ...................................................1.0mg
Selective supplement ...................................... 6.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems and Oxoid.
Selective Supplement:
Composition per 6.0mL:
Cefsulodin......................................................15.0mg
Irgasan..............................................................4.0mg
Novobiocin.......................................................2.5mg
Ethanol............................................................ 2.0mL
Preparation of Selective Supplement: Asepti
cally add components to 4.0mL of distilled/deionized
water and 2.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add selective supplement. Mix thoroughly. Pour into
sterile Petri dishes or leave in tubes.
Use: For the isolation and enumeration of Yersinia en
terocolitica from foods.
